Accurate quantification of recombinant proteins by amino acid analysis

How do I quantify my recombinant protein sample? Have you from time to time been questioning the quantitative data you get from Bradford,...

maj 15. 2014 | Thomas Kofoed | 0
GPMAW_lite

GPMAW lite – Protein Molecular Weight Calculator and much more

Simple bioinformatics calculations on specific protein sequences When working with proteins you often need to make simple bioinformatics calculations on a specific...

mar 10. 2014 | Thomas Kofoed | 0
N- and C-terminal Sequencing

Techniques for N- and C-terminal sequencing of proteins

Where does the amino acid sequence start and where does it end? The terminal amino acid sequences are important for the...

sep 16. 2013 | Ejvind Mørtz | 2
1D SDS PAGE

How to increase protein concentration before loading onto 1D SDS PAGE

Question: I have another question:  Is it ok if I precipitate my samples with TCA prior loading to the SDS-Gel? If not, how...

maj 16. 2012 | Ejvind Mørtz | 0

Identification of proteolytic degradation of recombinant protein

Question: Hi there, I purified a recombinant protein and analyzed it by 1D SDS PAGE. In addition to the intact protein...

maj 9. 2012 | Ejvind Mørtz | 2
billede2

Mass spectrometric identification of proteins detected in Western blots

Question: Dear Sir, We have an unknown protein recognized in western blot by autoantibodies that we need to identify. Is it...

maj 2. 2012 | Ejvind Mørtz | 0
billede9

What buffer to use for MALDI mass spectrometry analysis of a protein?

Question: What buffer should I use for MALDI Mass Spectrometry analysis of my protein sample? Answer: Although MALDI is more tolerant...

apr 28. 2010 | Ejvind Mørtz | 1

What does the Mascot Score mean?

Question: I just got my protein identification report. What does the Mascot Score mean? Answer: The Protein Identification report contains a list of proteins that have...

mar 9. 2010 | Ejvind Mørtz | 1

Preparing samples for N- and C-terminal sequencing by MALDI ISD?

Question: I am interested in your N and C-terminal sequencing using MALDI ISD. How do I prepare the sample for...

feb 22. 2010 | Ejvind Mørtz | 0

Identification of novel proteins not present in public databases

Question: My protein was analyzed using Alphalyse's Pick ‘n Post protein identification service. The sample gave very good MS data, but was not identified...

dec 2. 2009 | Ejvind Mørtz | 0
billede1

Databases and organisms available for mass spec protein ID service

Question: I would like to identify proteins from an organism where the genome has just been sequenced.  How many proteins are...

nov 16. 2009 | Ejvind Mørtz | 0

Useful Protein Molecular Weight Calculator

Protein molecular weight calculator The rule of thumb for conversion of microgram amounts into picomole amounts at different protein molecular...

nov 10. 2009 | Ejvind Mørtz | 0
billede10

Common protein contaminants observed in mass spectrometric protein ID

Questions: Is the keratin identified by the Pick ‘n Post protein ID service in my 1D gel band a contamination or...

nov 3. 2009 | Ejvind Mørtz | 0

Assessing the stability of a protein drug substance and formulated drug product

After protein purification, an important issue is the stability of a protein drug substance and formulated drug product. Stability assays...

okt 21. 2009 | Ejvind Mørtz | 1

How to optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing

How do I optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing? Our 12 kDa protein separates well on...

okt 13. 2009 | Ejvind Mørtz | 0

Mass Spectrometry for Host Cell Protein monitoring and control in pharmaceutical process development

Mass spectrometry based Host Cell Protein identification and quantification Here the Mass Spec Host Cell Protein (HCP) assay is applied for...

okt 2. 2009 | Ejvind Mørtz | 2
billede5

What to remember about Amino Acid Analysis of proteins and peptides

Amino acid analysis is a method to determine the absolute amounts of individual amino acids in a sample. The method...

sep 23. 2009 | Ejvind Mørtz | 1
1D 2D Gel electrophoresis

1D SDS PAGE versus 2D PAGE. I’m considering 2D electrophoresis, any recommendations?

Question: I’m interested in isolating and identifying cell surface receptor proteins, typically with high Mw, glycosylated and with transmembrane domains. I’m...

sep 18. 2009 | Ejvind Mørtz | 0

How should I prepare my samples for 2D electrophoresis, and how much protein should I load to get protein identification by mass spec?

How should I prepare my samples for 2D electrophoresis, and how much protein should I load to get protein ID...

sep 9. 2009 | Ejvind Mørtz | 0

Why is my protein’s N-terminal blocked?

N-terminal sequencing is a requirement according to the ICH Q6B Guideline for characterization of recombinant proteins for clinical testing, and...

aug 31. 2009 | Ejvind Mørtz | 2

N- and C-terminal protein sequencing using MALDI ISD

Structural characterization of purified natural and recombinant proteins traditionally includes N-terminal Edman sequencing to confirm 5-15 amino acid residues from...

aug 18. 2009 | Ejvind Mørtz | 0

Protocol for silver staining of gels optimized for mass spectrometry and protein identification

Silver staining is used for sensitive detection of proteins separated by 1D and 2D SDS PAGE with detection limits from...

aug 6. 2009 | Ejvind Mørtz | 1

What can I do to find out more about my protein?

What can I do to find out more about my protein? The first thing you should do is compare your sequence...

aug 5. 2009 | Ejvind Mørtz | 0

1D/2D PVDF blotting and staining protocols for N-terminal sequencing

For N-terminal Edman sequencing SDS PAGE separated proteins can be electroblotted onto PVDF membranes, the membranes stained and protein bands/spots...

jul 29. 2009 | Ejvind Mørtz | 0
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